Heterologous expression of the msp2 gene from Marasmius scorodonius
نویسندگان
چکیده
منابع مشابه
Antibiotics from basidiomycetes. X. Scorodonin, a new antibacterial and antifungal metabolite from Marasmius scorodonius (Fr.) Fr.
Scorodonin (1), a novel biologically active metabolite, was isolated from submerged cultures of the mushroom Marasmius scorodonius (FR.) FR. Its structure has been determined by chemical and physical methods. The antibiotic inhibits the growth of bacteria, yeasts, and filamentous fungi. In cells of the ascitic form of EHRLICH carcinoma the incorporation of thymidine and uridine into DNA and RNA...
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We have recently isolated a transmissible gastroenteritis virus (TGEV) infectious construct designated TGEV 1000 (B. Yount, K. M. Curtis, and R. S. Baric, J. Virol. 74:10600-10611, 2000). Using this construct, a recombinant TGEV was constructed that replaced open reading frame (ORF) 3A with a heterologous gene encoding green fluorescent protein (GFP). Following transfection of baby hamster kidn...
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Bacterial pathogens in the genera Anaplasma and Ehrlichia encode a protein superfamily, pfam01617, which includes the predominant outer membrane proteins (OMPs) of each species, major surface protein 2 (MSP2) and MSP3 of Anaplasma marginale and Anaplasma ovis, Anaplasma phagocytophilum MSP2 (p44), Ehrlichia chaffeensis p28-OMP, Ehrlichia canis p30, and Ehrlichia ruminantium MAP1, and has been s...
متن کاملVariability within the msp2 gene in populations of Anaplasma phagocythopilum.
Anaplasma phagocytophilum is an obligate intracellular bacterial parasite of verterbrate granulocytes. This bacterium is the aetiologic agent of human granulocytic anaplasmosis. The msp2 gene encoding major surface protein 2 is unique for Anaplasma and displays high antigenic variation. A fragment of the msp2 gene (334 bp) of A. phagocytophilum, amplified with DNA isolated from Ixodes ricinus, ...
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ژورنال
عنوان ژورنال: Archives of Microbiology
سال: 2009
ISSN: 0302-8933,1432-072X
DOI: 10.1007/s00203-009-0462-2